Dgcr8 Knockout cell line (HT22)

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Dgcr8 Knockout cell line (HT22) Order

Catalog#

YKO-M196

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Dgcr8 Knockout cell line (HT22)

Species

Mouse

Gene Name

Dgcr8

Gene ID

94223

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript Dgcr8-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Dgcr8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT22 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Dgcr8 gene knockout Dgcr8 Knockout cell line (HT22) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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